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氮吹仪聚糖的一级结构

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三、聚糖的一级结构及空间结构 !!氮吹仪聚糖的一级结构是指单糖的排列顺序及糖苷键的性质。糖苷键的性质包括单糖的连接位置, "或 #, !或 ",及吡喃糖和呋喃糖构型等。— $%& ,’(&’%—, —)(* 等非糖基团及蛋白质和脂质的结合位置也 在聚糖的一级结构中给予描述。 !!聚糖的二级结构涉及糖环构象、糖苷键旋转角度及各原子之间的相互作用等。由于糖样品不易纯化, 结构非常复杂,目前对于聚糖较高级的空间结构规律的认识尚不够清楚。但认识糖一级结构和空间结构 对了解糖的生物功能是非常重要的。 !!聚糖一级结构的测定可选择质谱(+$)、核磁共振( )+,)和 (-#’等多种仪器分析方法。特异水解 聚糖的糖苷酶(./012345637)是聚糖一级结构测定中常用的工具酶。糖苷酶包括内糖苷酶( 7852./012349 5637)和外糖苷酶(7:2./012345637)。两种聚糖水解酶作用的位点不同,内糖苷酶将糖链从内部特异的切 开,而外糖苷酶则从糖链的非还原端特异的水解糖基。图 &;是应用基质辅助激光离子解吸时间飞行 < 质谱(=6>?4: 63343>75 /637? [email protected]德州牛仔>428 4284A6>428 >4=7 2B B/4.C>,+D#"E F%G < +$)技术,对一个 )连接聚 糖经


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